Illumina 850K Methylation Microarray DNA Methylation Sequencing CapitalBio

As an important part of epigenomics, DNA methylation is involved in processes such as cell and tissue differentiation, development, aging, and environmental adaptation. Changes in DNA methylation patterns are related to multiple diseases, including cancer, mental disorders, diabetes, and cardiovascular disease (CVD). Based on the Illumina chip, CapitalBio Technology offers a comprehensive solution for DNA methylation detection and analysis, providing a one-stop service that covers screening and verification.

As an economic and efficient DNA methylation analysis high-throughput screening technology platform, the Illumina 850K methylation array can precisely detect methylation variations at the single-base level. This chip is a powerful tool for researching complex diseases such as tumors, respiratory system disorders, CVD, and mental diseases. It also serves as a high-throughput detection technology platform applicable to Epigenome-Wide Association Studies (EWAS).

CapitalBio Technology also offers methylation sequencing, methylation analysis, DNA methylation tests, and genetic methylation testing to support a wide range of research needs.

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Ewas

Regulation Of Gene Expression

Disease Molecular Marker

Research On Queue Sample

Research On Complex Disease Mechanism

Research On Disease Typing Or Prognosis Effects

illumina 850k Methylation Array Advantages Experiment Process Microarray Information Specifications

Widely Used: Our methylation technology platform is the most cited in scientific literature. It has been utilized to detect methylation in 10,181 samples by the Cancer Genome Atlas (TCGA) program in the United States and 9,246 samples by the International Cancer Genome Consortium (ICGC).

High Cost-Effectiveness: Our platform can analyze over 850,000 methylation sites with single-base resolution, allowing for the precise detection of methylation locations.

High Repeatability: Our DNA methylation analysis technology boasts a technical repeatability correlation (R²) greater than 0.98. Additionally, the correlation (R²) between the 450K intersection probe (850K vs. 450K) is also greater than 0.98.

Experienced: We have years of experience in providing DNA methylation chip services. Our project research areas include tumors, cardiovascular diseases, mental illnesses, immune diseases, drug epigenomes, reproduction and development, stem cells, and traditional Chinese medicine constitution.

Characteristic Analysis: Our data analysis team has extensive experience and has independently developed a illumina 850k methylation array chip analysis process. We offer tailored data analysis plans based on your experimental design. We also correct for cellular components to eliminate the interference of cellular heterogeneity, ensuring more rigorous and reliable results.

850k methylation array

DNA Extraction 2. QUBIT Quantification 3. Hydrosulphite conversion 4. Whole Genome Methylation amplification 5. 850k methylation array chip hybridization, elution extension and scanning 6. DNA methylation data analysis

Illumina 850k methylation array testing result

Illumina 850k Mmethylation Array Application cases:

DNA Methylation Signatures of Depressive Symptoms in Middle-aged and Elderly Persons: Meta-analysis of Multiethnic Epigenome-wide Studies

Genome-wide analysis of DNA methylation and acute coronary syndrome

Chip Name	Introduction to the chip
Illumina Infinium Methylation Epic BeadChip(850K)	>850,000 CpG loci: Cover >90% 450K methylation chip site; cover the promoter, CpG island, enhancer (>330,000 loci) and 99% of RefSeq gene; be compatible with FFPE samples

Sample Requirements	Purity: OD260/280 is within the range of 1.7-2.1
	DNA amount ≥2μg
	DNA Conc. ≥50ng/μL
	Integrity:main band of genome is clear and free from RNA contamination, protein contamination or degradation, with molecular weight ≥10Kb.

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