This test uses RT-qPCR technology to detect the expression of 16 tumor-related genes (proliferation, invasion, HER2, and hormone-related genes) and 5 reference genes and quantifies the test results as a Recurrence Score (RS) to predict 10-year long-term recurrence risk and chemotherapy benefit. This test can aid in the development of breast cancer treatment plans and avoid conditions such as undertreatment or overtreatment.