Single-Cell Sequencing CapitalBio

With the development of single-cell technology, the scope of research is no longer limited to the transcriptome but has expanded to multi-omics levels such as the genome, immunome, epigenome, and proteome. In complex biological processes, heterogeneity exists at multiple levels; cells with the same genes may have different patterns of DNA methylation, gene expression, and clonal expansion. To reveal deeper biological mechanisms, multi-omics techniques are required to more accurately classify them into different subgroups. CapitalBio uses the 10x Genomics Chromium X system to conduct single-cell sequencing services.

CapitalBio Technology offers comprehensive 10x Genomics single-cell sequencing services, utilizing advanced platforms like 10x Genomics sequencing to provide high-resolution insights into cellular heterogeneity and complex biological processes.

10x Genomics Single-Cell Sequencing Types

Scalable Single-Cell Research: CapitalBio utilizes 10x Genomics single-cell transcriptome sequencing technology to offer a more scalable platform for single-cell research. This high-throughput technique analyzes mRNA in multiple cells at the single-cell level.

Advanced Technology: The platform employs oil droplet encapsulation to label 500-10,000 single cells simultaneously. It then efficiently amplifies the trace mRNA in these labeled cells before performing high-throughput sequencing.

Solving Cell Heterogeneity: This technology effectively addresses the issue of cell heterogeneity that bulk tissue sample sequencing cannot resolve, aiding in the discovery of new cell types.

Wide Applications: Our platform is widely applicable in research areas such as map construction and developmental differentiation.

Single-cell Gene Expression With Cell Surface Protein

10x feature barcode technology, which can simultaneously detect gene expression and cell surface protein expression of each cell, which is conducive to better understanding of complex biological systems.

Single-Cell Transcriptome Sequencing

Through a large number of single-cell gene expression data, it can perform cell expression feature clustering, subgroup expression feature analysis, cell differentiation trajectory, cell-cell interactions, etc.

Single-Cell Immune Repertoire Sequencing

Through the 10x genomics platform, capitalbio technology can simultaneously detect the transcriptome information and tcr/bcr diversity information of each cell in one experiment.

Single-cell ATAC Sequencing

Capitalbio technology atac sequencing service uses the 10x genomics single-cell atac sequencing platform to detect the transposase-accessible chromatin at the single-cell level.

Spatial Multi-Omics Solutions

Capitalbio technology visium spatial gene expression service leverages the 10x genomics visium platform, combining microscopy imaging, targeted probe capture, microarray, and sequencing technologies to achieve histology and gene expression analysis.

Single-Cell CRISPR Screening

10x Genomics has developed a single-cell CRISPR screening platform, which uses high-throughput single-cell sequencing to obtain the gene expression profile and sgRNA expression of a single cell from the cells screened by CRISPR. The results of the CRISPR screen were tested at the level of phenotype.

Single-Cell Sequencing Platform

10x Genomics Chromium X is a novel high-throughput single-cell sequencing library construction and decoding system that can run up to millions of cells at a time, which can greatly reduce the cost of detection.

The X instrument has 16 channels, each channel can capture up to 20,000 cells, combined with 10x CellPlex multi-sample mixing technology, each channel can capture up to 60,000 cells, after removing the double cell rate, each chip can capture up to 750,000 cells single cell.

Single-Cell Sequencing CapitalBio

10x Genomics Single-Cell Sequencing Types

Single-Cell Sequencing Platform

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