Small RNA Sequencing CapitalBio

Small RNA is a kind of non-coding RNA with the length of 18-30nt. There are wide varieties, including miRNA, piRNA, endo-siRNA and snoRNA. miRNA, the most common research subject, is mainly concentrated in 20-24nt in length, and it is widely found in animals and plants. In animals, the main function of miRNA is to inhibit the translation of mRNA after transcription. In plants, the main function of miRNA is to degrade target mRNA directly. Illumina platform was used for sequencing, and miRNA analysis was carried out for reference genome samples.

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Profiling Known And Novel Transcripts And Expression Quantifications Of Small Rna

Predicting Targeting Genes Of Mirna Candidates

Identifying Tissue, Stage, Or Cell Type-specific Biomarkers For Cancer/disease Diagnostics And Classification

Discovering Regulatory Networks And Understanding The Mechanisms Of Tissue Or Organism Development Based On Transcript Profiles

Workflow Specifications

Platform	NovaSeq6000
Read Length	SE50
Data Output	>10M Reads
Sample Requirements	RNA amount > 2 ug
	RIN > 7
	Purity: A260/280 = 1.8-2.2, A260/230 ≥ 1.8
Data Analysis	Data QC Length Distribution Summary Common and Specific Sequence Summary Identification and Characterization of miRNAs Classification and Annotation of miRNA Quantification and Differential Expression Analysis Functional Enrichment Analysis

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